Journal: World Journal of Surgical Oncology

Article Title: Circ_0001741 regulates proliferation and invasion in ESCC via the miR-194-5p/E2F3 axis

doi: 10.1186/s12957-025-04124-2

Figure Lengend Snippet: E2F3 is a miR-194-5p target gene in TE2 and KYSE150 cells A Predicted partially complementary binding sequence between E2F3 mRNA and miR-194-5p B Dual-luciferase reporter assays confirming miR-194-5p binding to the E2F3 3’UTR. C Pull-down assay was used to confirm the interaction of E2F3 and miR-194-5p D Western blot analysis of E2F3 protein levels in TE2 cells after miR-194-5p overexpression. * P < 0.05

Article Snippet: Membranes were incubated overnight at 4 °C with E2F3 primary antibodies (Proteintech, China, Cat No. 27615-1-AP), followed by HRP-conjugated secondary antibodies (Proteintech, China).

Techniques: Binding Assay, Sequencing, Luciferase, Pull Down Assay, Western Blot, Over Expression

Journal: World Journal of Surgical Oncology

Article Title: Circ_0001741 regulates proliferation and invasion in ESCC via the miR-194-5p/E2F3 axis

doi: 10.1186/s12957-025-04124-2

Figure Lengend Snippet: E2F3 overexpression reverses the effects of circ_0001741 knockdown A E2F3 overexpression partially rescues the anti-invasive effects of si-circ_0001741 or miR-194-5p in TE2 and KYSE150 cells B E2F3 overexpression partially restores proliferation suppressed by si-circ_0001741 or miR-194-5p. * P < 0.05

Article Snippet: Membranes were incubated overnight at 4 °C with E2F3 primary antibodies (Proteintech, China, Cat No. 27615-1-AP), followed by HRP-conjugated secondary antibodies (Proteintech, China).

Techniques: Over Expression, Knockdown

Journal: Frontiers in Oncology

Article Title: Identification of MAD2L1 as a novel biomarker for hepatoblastoma through bioinformatics and machine learning approaches

doi: 10.3389/fonc.2025.1524714

Figure Lengend Snippet: MAD2L1 activates E2F transcription and regulates cell cycle regulators. (A, B) E2F transcription factor relative luciferase activity in dual-luciferase reporter gene assay. (C, D) The effect of MAD2L1 knockdown on Cyclin A2, E2F3 and Cyclin E1 expressions was detected by western blotting. (E, F) The effect of MAD2L1 knockdown on cell cycle was explored using a flow cytometry assay. *p < 0.05, **p < 0.01, ***p < 0.001, p ****<0.0001.

Article Snippet: Primary antibodies against MAD2L1 (1:600, Cat. No. 15283-1-AP, Proteintech), β-ACTIN (1:10,000, Cat. No. N901r, CellGene), β-Tubulin (1:1,500, Cat. No. E-AB-40518, Elabscience), GAPDH (1:3000, Cat. No.AF7021, AFFINITY), N-Cadherin Rabbit mAb (1:1000, Cat. No. A19083, ABclonal), E-Cadherin Rabbit mAb (1:1000, Cat. No. A20798, ABclonal), Cyclin A2 Polyclonal antibody (1:10000, Cat. No. 18202-1-AP, Proteintech), PCNA Polyclonal antibody (1:5000, Cat. No. 10205-2-AP, Proteintech), CyclinE1(1:1000, Cat. No. 11554-1-AP, proteintech) and E2F3(1:1000, Cat. No. 27615-1-AP, proteintech) were incubated overnight at 4°C.

Techniques: Luciferase, Activity Assay, Reporter Gene Assay, Knockdown, Western Blot, Flow Cytometry